Effect of azadirachtin on digestion, cytoskeletal structure and fecundity of Bemisia tabaci (Pages 13-18)

Abstract

Three digestive enzymes namely trypsin, alkaline phosphatase, lipase and two genes knottin 3 and vitellogenin were evaluated against whitefly after feeding with 5 ppm azadirachtin for 12 hours. Down regulated activity of all the three enzymes were observed. The transcript level of the two genes, related to reproductive system and cytoskeleton respectively were observed feeding with azadirachtin at similar concentration and exposure period as enzyme activity. It was observed that azadirachtin significantly inhibited the expression of both of the genes vitellogenin and knottin 3.